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Antitumor mechanisms of amino Acid hydroxyurea derivatives in the metastatic colon cancer model.

ABSTRACT: The paper presents a detailed study of the biological effects of two amino acid hydroxyurea derivatives that showed selective antiproliferative effects in vitro on the growth of human tumor cell line SW620. Tested compounds induced cell cycle perturbations and apoptosis. Proteins were identified by proteomics analyses using two-dimensional gel electrophoresis coupled to mass spectrometry, which provided a complete insight into the most probable mechanism of action on the protein level. Molecular targets for tested compounds were analyzed by cheminformatics tools. Zinc-dependent histone deacetylases were identified as potential targets responsible for the observed antiproliferative effect.

SUBMITTER: Saban N

PROVIDER: S-EPMC3876069 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Antitumor mechanisms of amino Acid hydroxyurea derivatives in the metastatic colon cancer model.

Saban Nina N Stepanić Višnja V Vučinić Srđan S Horvatić Anita A Cindrić Mario M Perković Ivana I Zorc Branka B Oršolić Nada N Mintas Mladen M Pavelić Krešimir K Pavelić Sandra Kraljević SK

International journal of molecular sciences 20131204 12

The paper presents a detailed study of the biological effects of two amino acid hydroxyurea derivatives that showed selective antiproliferative effects in vitro on the growth of human tumor cell line SW620. Tested compounds induced cell cycle perturbations and apoptosis. Proteins were identified by proteomics analyses using two-dimensional gel electrophoresis coupled to mass spectrometry, which provided a complete insight into the most probable mechanism of action on the protein level. Molecular ...[more]

PMID: 24304540

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