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Overexpression of an acidic endo-?-1,3-1,4-glucanase in transgenic maize seed for direct utilization in animal feed.


ABSTRACT: BACKGROUND: Incorporation of exogenous glucanase into animal feed is common practice to remove glucan, one of the anti-nutritional factors, for efficient nutrition absorption. The acidic endo-?-1,3-1,4-glucanase (Bgl7A) from Bispora sp. MEY-1 has excellent properties and represents a potential enzyme supplement to animal feed. METHODOLOGY/PRINCIPAL FINDINGS: Here we successfully developed a transgenic maize producing a high level of Bgl7AM (codon modified Bgl7A) by constructing a recombinant vector driven by the embryo-specific promoter ZM-leg1A. Southern and Western blot analysis indicated the stable integration and specific expression of the transgene in maize seeds over four generations. The ?-glucanase activity of the transgenic maize seeds reached up to 779,800 U/kg, about 236-fold higher than that of non-transgenic maize. The ?-glucanase derived from the transgenic maize seeds had an optimal pH of 4.0 and was stable at pH 1.0-8.0, which is in agreement with the normal environment of digestive tract. CONCLUSION/SIGNIFICANCE: Our study offers a transgenic maize line that could be directly used in animal feed without any glucanase production, purification and supplementation, consequently simplifying the feed enzyme processing procedure.

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3876984 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Overexpression of an acidic endo-β-1,3-1,4-glucanase in transgenic maize seed for direct utilization in animal feed.

Zhang Yuhong Y   Xu Xiaolu X   Zhou Xiaojin X   Chen Rumei R   Yang Peilong P   Meng Qingchang Q   Meng Kun K   Luo Huiying H   Yuan Jianhua J   Yao Bin B   Zhang Wei W  

PloS one 20131231 12


<h4>Background</h4>Incorporation of exogenous glucanase into animal feed is common practice to remove glucan, one of the anti-nutritional factors, for efficient nutrition absorption. The acidic endo-β-1,3-1,4-glucanase (Bgl7A) from Bispora sp. MEY-1 has excellent properties and represents a potential enzyme supplement to animal feed.<h4>Methodology/principal findings</h4>Here we successfully developed a transgenic maize producing a high level of Bgl7AM (codon modified Bgl7A) by constructing a re  ...[more]

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