Inferring the choreography of parental genomes during fertilization from ultralarge-scale whole-transcriptome analysis.

ABSTRACT: Fertilization precisely choreographs parental genomes by using gamete-derived cellular factors and activating genome regulatory programs. However, the mechanism remains elusive owing to the technical difficulties of preparing large numbers of high-quality preimplantation cells. Here, we collected >14 × 10(4) high-quality mouse metaphase II oocytes and used these to establish detailed transcriptional profiles for four early embryo stages and parthenogenetic development. By combining these profiles with other public resources, we found evidence that gene silencing appeared to be mediated in part by noncoding RNAs and that this was a prerequisite for post-fertilization development. Notably, we identified 817 genes that were differentially expressed in embryos after fertilization compared with

SUBMITTER: Park SJ 

PROVIDER: S-EPMC3877761 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature