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The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress.

ABSTRACT: Phosphorus (P) is essential for all living cells and organisms, and low-P stress is a major factor constraining plant growth and yield worldwide. In plants, P efficiency is a complex quantitative trait involving multiple genes, and the mechanisms underlying P efficiency are largely unknown. Combining linkage analysis, genome-wide and candidate-gene association analyses, and plant transformation, we identified a soybean gene related to P efficiency, determined its favorable haplotypes and developed valuable functional markers. First, six major genomic regions associated with P efficiency were detected by performing genome-wide associations (GWAs) in various environments. A highly significant region located on chromosome 8, qPE8, was identified by both GWAs and linkage mapping and explained 41% of the phenotypic variation. Then, a regional mapping study was performed with 40 surrounding markers in 192 diverse soybean accessions. A strongly associated haplotype (P = 10(-7)) consisting of the markers Sat_233 and BARC-039899-07603 was identified, and qPE8 was located in a region of approximately 250 kb, which contained a candidate gene GmACP1 that encoded an acid phosphatase. GmACP1 overexpression in soybean hairy roots increased P efficiency by 11-20% relative to the control. A candidate-gene association analysis indicated that six natural GmACP1 polymorphisms explained 33% of the phenotypic variation. The favorable alleles and haplotypes of GmACP1 associated with increased transcript expression correlated with higher enzyme activity. The discovery of the optimal haplotype of GmACP1 will now enable the accurate selection of soybeans with higher P efficiencies and improve our understanding of the molecular mechanisms underlying P efficiency in plants.

SUBMITTER: Zhang D

PROVIDER: S-EPMC3879153 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress.

Zhang Dan D Song Haina H Cheng Hao H Hao Derong D Wang Hui H Kan Guizhen G Jin Hangxia H Yu Deyue D

PLoS genetics 20140102 1

Phosphorus (P) is essential for all living cells and organisms, and low-P stress is a major factor constraining plant growth and yield worldwide. In plants, P efficiency is a complex quantitative trait involving multiple genes, and the mechanisms underlying P efficiency are largely unknown. Combining linkage analysis, genome-wide and candidate-gene association analyses, and plant transformation, we identified a soybean gene related to P efficiency, determined its favorable haplotypes and develop ...[more]

PMID: 24391523

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Project description:Southwest of China is one of the major soybean (Glycine max L.) production regions in China with low availability of soil phosphorus (P). Whereas little information is available on P-efficient soybean genotypes in this region, even though using P-efficient soybean genotypes is a sustainable P management strategy for enhancing yield and P use efficiency. To assess the genetic variation on P use efficiency, 274 soybean genotypes were employed to compare the yields and P acquisition potentials in the field. Additionally, 10 representational genotypes (5 P-efficient genotypes and 5 P-inefficient genotypes) were grown in hydroponic media containing low P treatment (0.05 mM L-1) and high P treatment (0.25 mM L-1) to further investigate P assimilation characteristics and the related mechanisms of P-efficient soybean genotypes. In the field trial, the models described the relationships between yield and seed P concentration (R2 = 0.85), shoot P accumulation (R2 = 0.84), HI (R2 = 0.82) well. The yield, seed P concentration and shoot P accumulation ranged from 5.5 to 36.0 g plant-1, from 0.045 to 0.93% and from 0.065 to 0.278 mg plant-1, respectively. In the hydroponic trial, P-efficient genotypes under low P treatment showed significantly better plant growth, P accumulation and root: shoot ratio than P-inefficient genotypes. Simultaneously, total root length, specific root length, root surface area and root volume of P-efficient were significantly greater than P-inefficient under low P treatment. Higher rate of organic acid exudation and acid phosphatase activities was observed in the P-efficient soybean genotypes under low P condition when compared to the P-inefficient soybean genotypes. It indicated that significant genetic variation for P use efficiency existed in this region, and the P-efficient soybean genotypes, especially E311 and E141, demonstrated great tolerance to P deficiency, which could be potential materials using in improving production and P use efficiency in low availability of soil P region.

Dataset Information

The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress.

Publications

The acid phosphatase-encoding gene GmACP1 contributes to soybean tolerance to low-phosphorus stress.

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