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ABSTRACT:
SUBMITTER: Kishikawa J
PROVIDER: S-EPMC3879562 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Kishikawa Jun-ichi J Nakanishi Atsuko A Furuike Shou S Tamakoshi Masatada M Yokoyama Ken K
The Journal of biological chemistry 20131118 1
Reduction of ATP hydrolysis activity of vacuolar-type ATPase/synthase (V0V1) as a result of ADP inhibition occurs as part of the normal mechanism of V0V1 of Thermus thermophilus but not V0V1 of Enterococcus hirae or eukaryotes. To investigate the molecular basis for this difference, domain-swapped chimeric V1 consisting of both T. thermophilus and E. hirae enzymes were generated, and their function was analyzed. The data showed that the interaction between the nucleotide binding and C-terminal d ...[more]