Ontology highlight
ABSTRACT:
SUBMITTER: Yang Y
PROVIDER: S-EPMC3880056 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Yang Yang Y Barendregt Arjan A Kamerling Johannis P JP Heck Albert J R AJ
Analytical chemistry 20131122 24
Taking chicken Ovalbumin as a prototypical example of a eukaryotic protein we use high-resolution native electrospray ionization mass spectrometry on a modified Exactive Orbitrap mass analyzer to qualitatively and semiquantitatively dissect 59 proteoforms in the natural protein. This variety is largely induced by the presence of multiple phosphorylation sites and a glycosylation site that we find to be occupied by at least 45 different glycan structures. Mass analysis of the intact protein in it ...[more]