Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.
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ABSTRACT:
SUBMITTER: Dallmann A
PROVIDER: S-EPMC3882076 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
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Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.
Dallmann Andre A Simon Bernd B Duszczyk Malgorzata M MM Kooshapur Hamed H Pardi Arthur A Bermel Wolfgang W Sattler Michael M
Angewandte Chemie (International ed. in English) 20130814 40
Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs. ...[more]
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