Ontology highlight
ABSTRACT:
SUBMITTER: Hohenstein KA
PROVIDER: S-EPMC3882114 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Hohenstein Kristi A KA Pyle April D AD Chern Jing Yi JY Lock Leslie F LF Donovan Peter J PJ
Stem cells (Dayton, Ohio) 20080306 6
High-efficiency genetic modification of human embryonic stem (hES) cells would enable manipulation of gene activity, routine gene targeting, and development of new human disease models and treatments. Chemical transfection, nucleofection, and electroporation of hES cells result in low transfection efficiencies. Viral transduction is efficient but has significant drawbacks. Here we describe techniques to transiently and stably express transgenes in hES cells with high efficiency using a widely av ...[more]