Ultraviolet laser-induced cross-linking in peptides.

ABSTRACT:

Rationale

The aim of this study was to demonstrate, and to characterize by high-resolution mass spectrometry that it is possible to preferentially induce covalent cross-links in peptides by using high-energy femtosecond ultraviolet (UV) laser pulses. The cross-link is readily formed only when aromatic amino acids are present in the peptide sequence.

Methods

Three peptides, xenopsin, angiotensin I, and interleukin, individually or in combination, were exposed to high-energy femtosecond UV laser pulses, either alone or in the presence of spin trapping molecules, the reaction products being characterized by high resolution mass spectrometry.

Results

High-resolution mass spectrometry and spin trapping strategies showed that cross-linking occurs readily, proceeds via a rad

SUBMITTER: Leo G 

PROVIDER: S-EPMC3882510 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature