Unknown

Dataset Information

0

Direct Ultraviolet Laser-Induced Reduction of Disulfide Bonds in Insulin and Vasopressin.


ABSTRACT: Ultraviolet (UV) light has been shown to induce reduction of disulfide bonds in proteins in solution. The photoreduction is proposed to be a result of electron donation from excited Tyr or Trp residues. In this work, a powerful UV femtosecond laser was used to generate photoreduced products, while the hypothesis of Tyr/Trp mediation was studied with spectroscopy and mass spectrometry. With limited irradiation times of 3 min or less at 280 nm, the laser-induced reduction in arginine vasopressin and human insulin led to significant yields of ?3% stable reduced product. The photogenerated thiols required acidic pH for stabilization, while neutral pH primarily caused scrambling and trisulfide formation. Interestingly, there was no direct evidence that Tyr/Trp mediation was a required criterion for the photoreduction of disulfide bonds. Intermolecular electron transfer remained a possibility for insulin but was ruled out for vasopressin. We propose that an additional mechanism should be increasingly considered in UV light-induced reduction of disulfide bonds in solution, in which a single UV photon is directly absorbed by the disulfide bond.

SUBMITTER: Gammelgaard SK 

PROVIDER: S-EPMC7161042 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Direct Ultraviolet Laser-Induced Reduction of Disulfide Bonds in Insulin and Vasopressin.

Gammelgaard Simon K SK   Petersen Steffen B SB   Haselmann Kim F KF   Nielsen Peter Kresten PK  

ACS omega 20200403 14


Ultraviolet (UV) light has been shown to induce reduction of disulfide bonds in proteins in solution. The photoreduction is proposed to be a result of electron donation from excited Tyr or Trp residues. In this work, a powerful UV femtosecond laser was used to generate photoreduced products, while the hypothesis of Tyr/Trp mediation was studied with spectroscopy and mass spectrometry. With limited irradiation times of 3 min or less at 280 nm, the laser-induced reduction in arginine vasopressin a  ...[more]

Similar Datasets

| S-EPMC3223978 | biostudies-literature
| S-EPMC9544300 | biostudies-literature
| S-EPMC3882510 | biostudies-literature
| S-EPMC2718267 | biostudies-literature
| S-EPMC4420526 | biostudies-literature
| S-EPMC7764730 | biostudies-literature
| S-EPMC4742852 | biostudies-other
| S-EPMC9418131 | biostudies-literature
| S-EPMC6926469 | biostudies-literature
| S-EPMC5725452 | biostudies-literature