Ontology highlight
ABSTRACT:
SUBMITTER: Gammelgaard SK
PROVIDER: S-EPMC7161042 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Gammelgaard Simon K SK Petersen Steffen B SB Haselmann Kim F KF Nielsen Peter Kresten PK
ACS omega 20200403 14
Ultraviolet (UV) light has been shown to induce reduction of disulfide bonds in proteins in solution. The photoreduction is proposed to be a result of electron donation from excited Tyr or Trp residues. In this work, a powerful UV femtosecond laser was used to generate photoreduced products, while the hypothesis of Tyr/Trp mediation was studied with spectroscopy and mass spectrometry. With limited irradiation times of 3 min or less at 280 nm, the laser-induced reduction in arginine vasopressin a ...[more]