Lipid in skeletal muscle myotubes is associated to the donors' insulin sensitivity and physical activity phenotypes.
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ABSTRACT:
SUBMITTER: Bajpeyi S
PROVIDER: S-EPMC3883809 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Publications
Lipid in skeletal muscle myotubes is associated to the donors' insulin sensitivity and physical activity phenotypes.
Bajpeyi Sudip S Myrland Cassandra K CK Covington Jeffrey D JD Obanda Diana D Cefalu William T WT Smith Steven R SR Rustan Arild C AC Ravussin Eric E
Obesity (Silver Spring, Md.) 20130910 2
<h4>Objective</h4>This study investigated the relationship between in vitro lipid content in myotubes and in vivo whole body phenotypes of the donors such as insulin sensitivity, intramyocellular lipids (IMCL), physical activity, and oxidative capacity.<h4>Design and methods</h4>Six physically active donors were compared to six sedentary lean and six T2DM. Lipid content was measured in tissues and myotubes by immunohistochemistry. Ceramides, triacylglycerols, and diacylglycerols (DAGs) were meas ...[more]
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