Ontology highlight
ABSTRACT:
SUBMITTER: Moreno-Mateos MA
PROVIDER: S-EPMC3884670 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Moreno-Mateos Miguel A MA Barragán Verónica V Torres Belén B Rodríguez-Mateo Cristina C Méndez-Vidal Cristina C Berezikov Eugene E Mudduluru Giridhar G Allgayer Heike H Pintor-Toro José A JA
RNA (New York, N.Y.) 20131015 12
MicroRNAs (miRNAs) have been widely studied in order to elucidate their biological functions. MicroRNA microarrays or miRNA overexpression libraries generated by synthesis and cloning of individual miRNAs have been used to study their different roles. In this work, we have developed a novel methodology to express mature miRNAs and other small RNAs from a double convergent RNA polymerase III promoter. We show that the generated miRNAs function similarly to those processed from primary transcripts ...[more]