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Thiols and selenols as electron-relay catalysts for disulfide-bond reduction.

ABSTRACT: Pass them on! Dithiobutylamine immobilized on a resin is a useful reagent for the reduction of disulfide bonds. Its ability to reduce a disulfide bond in a protein is enhanced greatly if used along with a soluble strained cyclic disulfide or mixed diselenide that relays electrons from the resin to the protein. This electron-relay catalysis system provides distinct advantages over the use of excess soluble reducing agent alone.

SUBMITTER: Lukesh JC

PROVIDER: S-EPMC3885359 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Thiols and selenols as electron-relay catalysts for disulfide-bond reduction.

Lukesh John C JC Vanveller Brett B Raines Ronald T RT

Angewandte Chemie (International ed. in English) 20131010 49

Pass them on! Dithiobutylamine immobilized on a resin is a useful reagent for the reduction of disulfide bonds. Its ability to reduce a disulfide bond in a protein is enhanced greatly if used along with a soluble strained cyclic disulfide or mixed diselenide that relays electrons from the resin to the protein. This electron-relay catalysis system provides distinct advantages over the use of excess soluble reducing agent alone. ...[more]

PMID: 24123634

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