Unknown

Dataset Information

0

Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase.


ABSTRACT: We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.

SUBMITTER: Ryder E 

PROVIDER: S-EPMC3890051 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8664424 | biostudies-literature
| S-EPMC2757265 | biostudies-literature
| S-EPMC4724263 | biostudies-literature
| S-EPMC7195411 | biostudies-literature
| S-EPMC1346652 | biostudies-literature
| S-EPMC4850732 | biostudies-literature
| S-BSST580 | biostudies-other
| S-EPMC7852330 | biostudies-literature
| S-EPMC9731692 | biostudies-literature
| S-EPMC3344920 | biostudies-literature