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Discovery of a structural-element specific G-quadruplex "light-up" probe.

ABSTRACT: The development of a fluorescent probe capable of detecting and distinguishing the wide diversity of G-quadruplex structures is particularly challenging. Herein, we report a novel BODIPY-based fluorescent sensor (GQR) that shows unprecedented selectivity to parallel-stranded G-quadruplexes with exposed ends and four medium grooves. Mechanistic studies suggest that GQR associates with G-quadruplex grooves close to the end of the tetrad core, which may explain the dye's specificity to only a subset of parallel structures. This specific recognition favours the disaggregation of GQR in aqueous solutions thereby recovering the inherent fluorescence of the dye. Due to its unique features, GQR represents a valuable tool for basic biological research and the rapid discovery of novel, specific ligands that target similar structural features of G-quadruplexes.

SUBMITTER: Zhang L

PROVIDER: S-EPMC3895904 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Discovery of a structural-element specific G-quadruplex "light-up" probe.

Zhang Liyun L Er Jun Cheng JC Ghosh Krishna Kanta KK Chung Wan Jun WJ Yoo Jaeduk J Xu Wang W Zhao Wei W Phan Anh Tuân AT Chang Young-Tae YT

Scientific reports 20140120

The development of a fluorescent probe capable of detecting and distinguishing the wide diversity of G-quadruplex structures is particularly challenging. Herein, we report a novel BODIPY-based fluorescent sensor (GQR) that shows unprecedented selectivity to parallel-stranded G-quadruplexes with exposed ends and four medium grooves. Mechanistic studies suggest that GQR associates with G-quadruplex grooves close to the end of the tetrad core, which may explain the dye's specificity to only a subse ...[more]

PMID: 24441075

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