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A general fluorescent light-up probe for staining and quantifying protein.

ABSTRACT: Proteins are the primary functional agents in all cellular processes, facilitating various functions such as enzymes and structure-forming or signal-transducing molecules. In this work, we report a fluorescent dye, PyMDI-Zn, which could specifically bind with proteins and provide a red-shifted fluorescent emission. The visual analysis of protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis could be realized in 5 min by using PyMDI-Zn as a light-up dye. Based on its cell penetration and low toxicity, PyMDI-Zn could also be applied to locate protein-rich regions and organelles in live cell imaging. Moreover, the direct protein quantitation can be realized based on PyMDI-Zn, providing a method of screening for food adulteration by nitrogen-rich compounds.

SUBMITTER: Zou J

PROVIDER: S-EPMC6731743 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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A general fluorescent light-up probe for staining and quantifying protein.

Zou Jiawei J Chen Gangyi G Du Feng F Yuan Yi Y Huang Xin X Dong Juan J Xie Kexin K Cui Xin X Tang Zhuo Z

Royal Society open science 20190828 8

Proteins are the primary functional agents in all cellular processes, facilitating various functions such as enzymes and structure-forming or signal-transducing molecules. In this work, we report a fluorescent dye, PyMDI-Zn, which could specifically bind with proteins and provide a red-shifted fluorescent emission. The visual analysis of protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis could be realized in 5 min by using PyMDI-Zn as a light-up dye. Based on its cell penetrati ...[more]

PMID: 31598246

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