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ABSTRACT:
SUBMITTER: Nakabayashi Y
PROVIDER: S-EPMC3896174 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Nakabayashi Yu Y Kawashima Satoshi S Enomoto Takemi T Seki Masayuki M Horikoshi Masami M
Proceedings of the National Academy of Sciences of the United States of America 20131227 2
Currently, there is no method to distinguish between the roles of a subunit in one multisubunit protein complex from its roles in other complexes in vivo. This is because a mutation in a common subunit will affect all complexes containing that subunit. Here, we describe a unique method to discriminate between the functions of a common subunit in different multisubunit protein complexes. In this method, a common subunit in a multisubunit protein complex is genetically fused to a subunit that is s ...[more]