Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering.

ABSTRACT: Transcription activator-like (TAL) effector nucleases (TALENs) have enabled the introduction of targeted genetic alterations into a broad range of cell lines and organisms. These customizable nucleases are comprised of programmable sequence-specific DNA-binding modules derived from TAL effector proteins fused to the non-specific FokI cleavage domain. Delivery of these nucleases into cells has proven challenging as the large size and highly repetitive nature of the TAL effector DNA-binding domain precludes their incorporation into many types of viral vectors. Furthermore, viral and non-viral gene delivery methods carry the risk of insertional mutagenesis and have been shown to increase the off-target activity of site-specific nucleases. We previously demonstrated that direct delivery of zin

SUBMITTER: Liu J 

PROVIDER: S-EPMC3896395 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature