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Tunable membranes for free-flow zone electrophoresis in PDMS microchip using guided self-assembly of silica microbeads.

ABSTRACT: In this paper, we evaluate the strategy of using self-assembled microbeads to build a robust and tunable membrane for free-flow zone electrophoresis in a PDMS microfluidic chip. To fabricate a porous membrane as a salt bridge for free-flow zone electrophoresis, we used silica or polystyrene microbeads between 3-6 ?m in diameter and packed them inside a microchannel. After complete evaporation, we infiltrated the porous microbead structure with a positively or negatively charged hydrogel to modify its surface charge polarity. Using this device, we demonstrated binary sorting (separation of positive and negative species at a given pH) of peptides and dyes in standard buffer systems without using sheath flows. The sample loss during sorting could be minimized by using ion selectivity of hydrogel-infiltrated microbead membranes. Our fabrication method enables building a robust membrane for pressure-driven free-flow zone electrophoresis with tunable pore size as well as surface charge polarity.

SUBMITTER: Song YA

PROVIDER: S-EPMC3896612 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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2024-10-15 | PXD051862 | Pride

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