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Comparison of separation modes for microchip electrophoresis of proteins.

ABSTRACT: Separation of a set of model proteins was tested on a microchip electrophoresis analytical platform capable of sample injection by two different electrokinetic mechanisms. A range of separation modes-microchip capillary zone electrophoresis, microchip micellar electrokinetic chromatography, and nanoparticle-based sieving-was tested on glass and polydimethylsiloxane/glass microchips and with silica-nanoparticle colloidal arrays. The model proteins calmodulin (18 kiloDalton), bovine serum albumin (66 kDa), and concanavalin (106 kDa) were labeled with Alexa Fluor 647 for laser-induced fluorescence detection. The best separation and resolution were obtained in a silica-nanoparticle colloidal array chip.

SUBMITTER: Samarasinghe TN

PROVIDER: S-EPMC8053037 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Comparison of separation modes for microchip electrophoresis of proteins.

Samarasinghe Thushara N TN Zeng Yong Y Johnson Carey K CK

Journal of separation science 20201213 3

Separation of a set of model proteins was tested on a microchip electrophoresis analytical platform capable of sample injection by two different electrokinetic mechanisms. A range of separation modes-microchip capillary zone electrophoresis, microchip micellar electrokinetic chromatography, and nanoparticle-based sieving-was tested on glass and polydimethylsiloxane/glass microchips and with silica-nanoparticle colloidal arrays. The model proteins calmodulin (18 kiloDalton), bovine serum albumin ...[more]

PMID: 33226183

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