Ontology highlight
ABSTRACT:
SUBMITTER: Berry SM
PROVIDER: S-EPMC3897128 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Berry Scott M SM Chin Emily N EN Jackson Shawn S SS Strotman Lindsay N LN Goel Mohit M Thompson Nancy E NE Alexander Caroline M CM Miyamoto Shigeki S Burgess Richard R RR Beebe David J DJ
Analytical biochemistry 20131109
Biological mechanisms are often mediated by transient interactions between multiple proteins. The isolation of intact protein complexes is essential to understanding biochemical processes and an important prerequisite for identifying new drug targets and biomarkers. However, low-affinity interactions are often difficult to detect. Here, we use a newly described method called immiscible filtration assisted by surface tension (IFAST) to isolate proteins under defined binding conditions. This metho ...[more]