Unknown

Dataset Information

0

Toxicity assessment of silica coated iron oxide nanoparticles and biocompatibility improvement by surface engineering.

ABSTRACT: We have studied in vitro toxicity of iron oxide nanoparticles (NPs) coated with a thin silica shell (Fe3O4/SiO2 NPs) on A549 and HeLa cells. We compared bare and surface passivated Fe3O4/SiO2 NPs to evaluate the effects of the coating on the particle stability and toxicity. NPs cytotoxicity was investigated by cell viability, membrane integrity, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) assays, and their genotoxicity by comet assay. Our results show that NPs surface passivation reduces the oxidative stress and alteration of iron homeostasis and, consequently, the overall toxicity, despite bare and passivated NPs show similar cell internalization efficiency. We found that the higher toxicity of bare NPs is due to their stronger in-situ degradation, with larger intracellular release of iron ions, as compared to surface passivated NPs. Our results indicate that surface engineering of Fe3O4/SiO2 NPs plays a key role in improving particles stability in biological environments reducing both cytotoxic and genotoxic effects.

SUBMITTER: Malvindi MA 

PROVIDER: S-EPMC3897540 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Toxicity assessment of silica coated iron oxide nanoparticles and biocompatibility improvement by surface engineering.

Malvindi Maria Ada MA   De Matteis Valeria V   Galeone Antonio A   Brunetti Virgilio V   Anyfantis George C GC   Athanassiou Athanassia A   Cingolani Roberto R   Pompa Pier Paolo PP  

PloS one 20140121 1

We have studied in vitro toxicity of iron oxide nanoparticles (NPs) coated with a thin silica shell (Fe3O4/SiO2 NPs) on A549 and HeLa cells. We compared bare and surface passivated Fe3O4/SiO2 NPs to evaluate the effects of the coating on the particle stability and toxicity. NPs cytotoxicity was investigated by cell viability, membrane integrity, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) assays, and their genotoxicity by comet assay. Our results show that NPs surface p  ...[more]

Similar Datasets

Unknown In vitro biocompatibility study of sub-5 nm silica-coated magnetic iron oxide fluorescent nanoparticles for potential biomedical application.
| S-EPMC5395943 | biostudies-literature
Unknown Preparation of Scalable Silica-Coated Iron Oxide Nanoparticles for Nanowarming.
| S-EPMC7029634 | biostudies-literature
Unknown Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation.
| S-EPMC7022723 | biostudies-literature
Unknown <i>In vitro</i> toxicity assessment of chitosan oligosaccharide coated iron oxide nanoparticles.
| S-EPMC5598369 | biostudies-literature
Unknown Silica Coated Iron/Iron Oxide Nanoparticles as a Nano-Platform for T<sub>2</sub> Weighted Magnetic Resonance Imaging.
| S-EPMC6943426 | biostudies-literature
Unknown Engineering Iron Oxide Nanoparticles for Clinical Settings.
| S-EPMC6029241 | biostudies-literature
Transcriptomics Transcriptomic fingerprints of C. elegans exposed to citrate coated superparamagnetic iron oxide nanoparticles (C-SPIONs) and to superparamagnetic iron oxide nanoparticles coated with a monolayer of bovine serum albumin (BSA-SPIONs)
2018-01-05 | GSE93188 | GEO
Unknown Compact zwitterion-coated iron oxide nanoparticles for biological applications.
| S-EPMC3278278 | biostudies-literature
Unknown Micellar Iron Oxide Nanoparticles Coated with Anti-Tumor Glycosides.
| S-EPMC6116232 | biostudies-literature
Unknown Iodinated oil-loaded, fluorescent mesoporous silica-coated iron oxide nanoparticles for magnetic resonance imaging/computed tomography/fluorescence trimodal imaging.
| S-EPMC4039419 | biostudies-literature