The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation.
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ABSTRACT:
SUBMITTER: Hoogendoorn E
PROVIDER: S-EPMC3900998 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Publications
The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation.
Hoogendoorn Eelco E Crosby Kevin C KC Leyton-Puig Daniela D Breedijk Ronald M P RM Jalink Kees K Gadella Theodorus W J TW Postma Marten M
Scientific reports 20140124
The quality of super resolution images obtained by stochastic single-molecule microscopy critically depends on image analysis algorithms. We find that the choice of background estimator is often the most important determinant of reconstruction quality. A variety of techniques have found use, but many have a very narrow range of applicability depending upon the characteristics of the raw data. Importantly, we observe that when using otherwise accurate algorithms, unaccounted background components ...[more]
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