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Chemical synthesis of U1 snRNA derivatives.

ABSTRACT: U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the binding affinity of the modified U1 snRNA with an ethylene glycol linkage to snurportin 1 (nuclear import adaptor) was as high as that of the unmodified RNA.

SUBMITTER: Ohkubo A

PROVIDER: S-EPMC3901379 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Chemical synthesis of U1 snRNA derivatives.

Ohkubo Akihiro A Kondo Yasushi Y Suzuki Makoto M Kobayashi Haruki H Kanamori Takashi T Masaki Yoshiaki Y Seio Kohji K Nagai Kiyoshi K Sekine Mitsuo M

Organic letters 20130816 17

U1 snRNA is an interesting biological tool for splicing correction and regulation of gene expression. However, U1 snRNA has never been chemically synthesized. In this study, the first chemical synthesis of U1snRNA and its analogues was carried out. Moreover, it was found that the binding affinity of the modified U1 snRNA with an ethylene glycol linkage to snurportin 1 (nuclear import adaptor) was as high as that of the unmodified RNA. ...[more]

PMID: 23952175

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