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On-chip electrophoresis in supported lipid bilayer membranes achieved using low potentials.

ABSTRACT: A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25-1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.

SUBMITTER: van Weerd J

PROVIDER: S-EPMC3901391 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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On-chip electrophoresis in supported lipid bilayer membranes achieved using low potentials.

van Weerd Jasper J Krabbenborg Sven O SO Eijkel Jan J Karperien Marcel M Huskens Jurriaan J Jonkheijm Pascal P

Journal of the American Chemical Society 20131219 1

A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying ...[more]

PMID: 24345193

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