Dataset Information

Effect of maraviroc intensification on HIV-1-specific T cell immunity in recently HIV-1-infected individuals.

ABSTRACT:

Background

The effect of maraviroc on the maintenance and the function of HIV-1-specific T cell responses remains unknown.

Methods

Subjects recently infected with HIV-1 were randomized to receive anti-retroviral treatment with or without maraviroc intensification for 48 weeks, and were monitored up to week 60. PBMC and in vitro-expanded T cells were tested for responses to the entire HIV proteome by ELISpot analyses. Intracellular cytokine staining assays were conducted to monitor the (poly)-functionality of HIV-1-specific T cells. Analyses were performed at baseline and week 24 after treatment start, and at week 60 (3 months after maraviroc discontinuation).

Results

Maraviroc intensification was associated with a slower decay of virus-specific T cell responses over time compared to the non-intensified regimen in both direct ex-vivo as well as in in-vitro expanded cells. The effector function profiles of virus-specific CD8? T cells were indistinguishable between the two arms and did not change over time between the groups.

Conclusions

Maraviroc did not negatively impact any of the measured parameters, but was rather associated with a prolonged maintenance of HIV-1-specific T cell responses. Maraviroc, in addition to its original effect as viral entry inhibitor, may provide an additional benefit on the maintenance of virus-specific T cells which may be especially important for future viral eradication strategies.

SUBMITTER: Kawana-Tachikawa A

PROVIDER: S-EPMC3903883 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Publications

Effect of maraviroc intensification on HIV-1-specific T cell immunity in recently HIV-1-infected individuals.

Kawana-Tachikawa Ai A Llibre Josep M JM Bravo Isabel I Escrig Roser R Mothe Beatriz B Puig Jordi J Puertas Maria C MC Martinez-Picado Javier J Blanco Julia J Manzardo Christian C Miro Jose M JM Iwamoto Aikichi A Pozniak Anton L AL Gatell Jose M JM Clotet Bonaventura B Brander Christian C

PloS one 20140127 1

<h4>Background</h4>The effect of maraviroc on the maintenance and the function of HIV-1-specific T cell responses remains unknown.<h4>Methods</h4>Subjects recently infected with HIV-1 were randomized to receive anti-retroviral treatment with or without maraviroc intensification for 48 weeks, and were monitored up to week 60. PBMC and in vitro-expanded T cells were tested for responses to the entire HIV proteome by ELISpot analyses. Intracellular cytokine staining assays were conducted to monitor ...[more]

PMID: 24475275

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Project description:BackgroundYunnan has the longest endured Human Immunodeficiency Virus-1 (HIV-1) epidemic in China, and the genetic diversity of HIV-1 constitutes an essential characteristic of molecular epidemiology in this region. To obtain a more comprehensive picture of the dynamic changes in Yunnan's HIV-1 epidemic, a cross-sectional molecular epidemiological investigation was carried out among recently infected individuals.Methodology/principal findingsWe sequenced partial gag (HXB2?781-1861) and env (HXB2?7002-7541) genes from 308 plasma samples of recently infected patients. With phylogenetic analysis, 130 specimens generated interpretable genotyping data. We found that the circulating genotypes included: CRF08_BC (40.8%), unique recombinant forms (URFs, 27.7%), CRF01_AE (18.5%), CRF07_BC (9.2%), subtype B (2.3%) and C (1.5%). CRF08_BC was the most common genotype, and was predominant in both intravenous drug users (IDUs) and heterosexually transmitted populations. CRF08_BC and CRF07_BC still predominated in eastern Yunnan, but CRF08_BC showed increasing prevalence in western Yunnan. Strikingly, the URFs raised dramatically in most regions of Yunnan. Seven different types of URFs were detected from 12 prefectures, suggesting that complicated and frequent recombination is a salient feature of Yunnan's HIV-1 epidemic. Among URFs, two BC clusters with distinctive recombination patterns might be potential new CRF_BCs. CRF01_AE was no longer confined to the prefectures bordering Myanmar, and had spread to the eastern part of Yunnan, especially the capital city of Kunming, with a large number of infections in the transient population. The ratios of the main genotypes showed no statistical differences between infected IDUs and heterosexually transmitted infections.Conclusions/significanceThe changing patterns of the dominant HIV-1 genotypes in Yunnan indicate the complex evolving dynamic nature of the epidemic. Understanding new trends in molecular epidemiology of HIV-1 infection is critical for adjusting current prevention strategies and vaccine development in Yunnan.

Dataset Information

Effect of maraviroc intensification on HIV-1-specific T cell immunity in recently HIV-1-infected individuals.

Background

Methods

Results

Conclusions

Publications

Effect of maraviroc intensification on HIV-1-specific T cell immunity in recently HIV-1-infected individuals.

Similar Datasets

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