Ontology highlight
ABSTRACT:
SUBMITTER: Ghosh D
PROVIDER: S-EPMC3905571 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Ghosh Debadyuti D Kohli Aditya G AG Moser Felix F Endy Drew D Belcher Angela M AM
ACS synthetic biology 20120924 12
M13 bacteriophage is a well-characterized platform for peptide display. The utility of the M13 display platform is derived from the ability to encode phage protein fusions with display peptides at the genomic level. However, the genome of the phage is complicated by overlaps of key genetic elements. These overlaps directly couple the coding sequence of one gene to the coding or regulatory sequence of another, making it difficult to alter one gene without disrupting the other. Specifically, overl ...[more]