Dataset Information

Decreased expression of GATA2 promoted proliferation, migration and invasion of HepG2 in vitro and correlated with poor prognosis of hepatocellular carcinoma.

ABSTRACT:

Background

GATA family of transcription factors are critical for organ development and associated with progression of various cancer types. However, their expression patterns and prognostic values for hepatocellular carcinoma (HCC) are still largely unknown.

Methods

Expression of GATA transcription factors in HCC cell lines and tissues (n?=?240) were evaluated by RT-qPCR, western blot and immunohistochemistry. Cellular proliferation, migration and invasion of HepG2 was evaluated by CCK-8 kit, scratch wound assay and transwell matrigel invasion assay, respectively.

Results

GATA2 expression was decreased in HCC cell lines (p?=?0.056 for mRNA, p?=?0.040 for protein) and tissues (p?=?1.27E-25) compared with normal hepatocytes. Decreased expression of intratumoral GATA2 protein significantly correlated with elevated alpha feto-protein (p?=?2.7E-05), tumor size >5 cm (p?=?0.049), absence of tumor capsule (p?=?0.002), poor differentiation (p?=?0.005), presence of tumor thrombi (p?=?0.005) and advanced TNM stage (p?=?0.001) and was associated with increased recurrence rate and decreased overall survival rate by univariate (p?=?1.6E-04 for TTR, p?=?1.7E-04 for OS) and multivariate analyses (HR?=?0.63, 95% CI?=?0.43-0.90, p?=?0.012 for TTR; HR?=?0.67, 95% CI?=?0.47-0.95, p?=?0.026 for OS). RNAi-mediated knockdown of GATA2 expression significantly enhanced proliferation, migration and invasion of HepG2 cell in vitro.

Conclusions

Decreased expression of hematopoietic factor GATA2 was associated with poor prognosis of HCC following resection.

SUBMITTER: Li YW

PROVIDER: S-EPMC3907524 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Publications

Decreased expression of GATA2 promoted proliferation, migration and invasion of HepG2 in vitro and correlated with poor prognosis of hepatocellular carcinoma.

Li Yi-Wei YW Wang Jia-Xing JX Yin Xin X Qiu Shuang-Jian SJ Wu Han H Liao Rui R Yi Yong Y Xiao Yong-Sheng YS Zhou Jian J Zhang Bo-Heng BH Fan Jia J

PloS one 20140130 1

<h4>Background</h4>GATA family of transcription factors are critical for organ development and associated with progression of various cancer types. However, their expression patterns and prognostic values for hepatocellular carcinoma (HCC) are still largely unknown.<h4>Methods</h4>Expression of GATA transcription factors in HCC cell lines and tissues (n = 240) were evaluated by RT-qPCR, western blot and immunohistochemistry. Cellular proliferation, migration and invasion of HepG2 was evaluated b ...[more]

PMID: 24498120

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Project description:BackgroundPrevious reports show that phospholipase C epsilon-1 (PLCE1) expression is positively correlated with esophageal squamous cell carcinoma and gastric cardia adenocarcinomas; however, the expression of PLCE1 in hepatocellular carcinoma (HCC) and its correlation with clinical outcome still remain unclear. The aim of this study was to explore the expression of PLCE1 in HCC tissue and to determine whether PLCE1 was a prognostic factor for HCC patients.Materials and methodsPLCE1 levels in 20 paired HCC tissues and corresponding paracarcinomatous tissues was investigated by quantitative real-time polymerase chain reaction and Western blot assays. In addition, protein levels of PLCE1 in one normal liver epithelial cell and four HCC cell lines were examined using Western blot assay. Moreover, immunohistochemistry was applied to determine the expression of PLCE1 in HCC and corresponding surrounding tissues from 90 patients. Statistical analyses were used to examine the association between PLCE1 levels and clinicopathological features.ResultsWe found that the expression of PLCE1 in tumor tissues was significantly lower than those in paracarcinomatous tissues at both mRNA and protein levels (P<0.05). We also determined that PLCE1 protein expression levels were lower in HCC cell lines than normal liver epithelial cells (P<0.05). Notably, immunohistochemical assay showed that PLCE1 expression was significantly low in HCC tissues compared with the adjacent normal liver tissues (40% vs 18.9%; P<0.05). Besides, PLCE1 levels were negatively correlated with tumor capsulae, vascular invasion, Edmondson grade, alpha-fetoprotein, and tumor-node-metastasis stage (P<0.05). Univariate analysis revealed that lower level expression of PLCE1 was significantly associated with poorer overall survival (OS) rate (P<0.001) and disease-free survival rate (P<0.001). Multivariate analysis revealed that low PLCE1 level was an independent poor prognostic factor of OS and recurrence-free survival (P<0.001 and P=0.003, respectively).ConclusionIn brief, our results revealed that decreased PLCE1 expression was associated with tumor progression in HCC and may function as a promising biomarker for HCC prognosis.

Dataset Information

Decreased expression of GATA2 promoted proliferation, migration and invasion of HepG2 in vitro and correlated with poor prognosis of hepatocellular carcinoma.

Background

Methods

Results

Conclusions

Publications

Decreased expression of GATA2 promoted proliferation, migration and invasion of HepG2 in vitro and correlated with poor prognosis of hepatocellular carcinoma.

Similar Datasets

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