Antibiotic resistance genes in the bacteriophage DNA fraction of human fecal samples.
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ABSTRACT:
SUBMITTER: Quiros P
PROVIDER: S-EPMC3910789 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Antibiotic resistance genes in the bacteriophage DNA fraction of human fecal samples.
Quirós Pablo P Colomer-Lluch Marta M Martínez-Castillo Alexandre A Miró Elisenda E Argente Marc M Jofre Juan J Navarro Ferran F Muniesa Maite M
Antimicrobial agents and chemotherapy 20131028 1
A group of antibiotic resistance genes (ARGs) (blaTEM, blaCTX-M-1, mecA, armA, qnrA, and qnrS) were analyzed by real-time quantitative PCR (qPCR) in bacteriophage DNA isolated from feces from 80 healthy humans. Seventy-seven percent of the samples were positive in phage DNA for one or more ARGs. blaTEM, qnrA, and, blaCTX-M-1 were the most abundant, and armA, qnrS, and mecA were less prevalent. Free bacteriophages carrying ARGs may contribute to the mobilization of ARGs in intra- and extraintesti ...[more]
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