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DNA in antibiotic preparations: absence of intact resistance genes.

ABSTRACT: Fragments of erm(E2), otrA, and aph(6) shorter than 400 bp and producer strain-specific rRNA genes were amplified from various antibiotics. The amount of genetic material and the sizes of amplicons recovered from murine feces after oral administration of a beta-lactamase-encoding plasmid indicated substantial DNA degradation in the mammalian gastrointestinal tract. These observations imply that antibiotics are no major source for horizontal resistance gene transfer in clinical settings.

SUBMITTER: Woegerbauer M

PROVIDER: S-EPMC1140494 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature

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DNA in antibiotic preparations: absence of intact resistance genes.

Woegerbauer Markus M Lagler Heimo H Graninger Wolfgang W Burgmann Heinz H

Antimicrobial agents and chemotherapy 20050601 6

Fragments of erm(E2), otrA, and aph(6) shorter than 400 bp and producer strain-specific rRNA genes were amplified from various antibiotics. The amount of genetic material and the sizes of amplicons recovered from murine feces after oral administration of a beta-lactamase-encoding plasmid indicated substantial DNA degradation in the mammalian gastrointestinal tract. These observations imply that antibiotics are no major source for horizontal resistance gene transfer in clinical settings. ...[more]

PMID: 15917552

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