Ontology highlight
ABSTRACT:
SUBMITTER: Chen Z
PROVIDER: S-EPMC3911046 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Chen Zhen Z Bommareddy Rajesh Reddy RR Frank Doinita D Rappert Sugima S Zeng An-Ping AP
Applied and environmental microbiology 20131213 4
Allosteric regulation of phosphoenolpyruvate carboxylase (PEPC) controls the metabolic flux distribution of anaplerotic pathways. In this study, the feedback inhibition of Corynebacterium glutamicum PEPC was rationally deregulated, and its effect on metabolic flux redistribution was evaluated. Based on rational protein design, six PEPC mutants were designed, and all of them showed significantly reduced sensitivity toward aspartate and malate inhibition. Introducing one of the point mutations (N9 ...[more]