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Diagnosis of prosthetic joint infection by use of PCR-electrospray ionization mass spectrometry.

ABSTRACT: We compared PCR-electrospray ionization mass spectrometry (PCR-ESI/MS) to culture using sonicate fluid from 431 subjects with explanted knee (n = 270) or hip (n = 161) prostheses. Of these, 152 and 279 subjects had prosthetic joint infection (PJI) and aseptic failure, respectively. The sensitivities for detecting PJI were 77.6% for PCR-ESI/MS and 69.7% for culture (P = 0.0105). The specificities were 93.5 and 99.3%, respectively (P = 0.0002).

SUBMITTER: Greenwood-Quaintance KE

PROVIDER: S-EPMC3911314 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Diagnosis of prosthetic joint infection by use of PCR-electrospray ionization mass spectrometry.

Greenwood-Quaintance Kerryl E KE Uhl James R JR Hanssen Arlen D AD Sampath Rangarajan R Mandrekar Jayawant N JN Patel Robin R

Journal of clinical microbiology 20131127 2

We compared PCR-electrospray ionization mass spectrometry (PCR-ESI/MS) to culture using sonicate fluid from 431 subjects with explanted knee (n = 270) or hip (n = 161) prostheses. Of these, 152 and 279 subjects had prosthetic joint infection (PJI) and aseptic failure, respectively. The sensitivities for detecting PJI were 77.6% for PCR-ESI/MS and 69.7% for culture (P = 0.0105). The specificities were 93.5 and 99.3%, respectively (P = 0.0002). ...[more]

PMID: 24478503

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