A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus.
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ABSTRACT:
SUBMITTER: Pickering J
PROVIDER: S-EPMC3911354 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
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A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus.
Pickering Janessa J Binks Michael J MJ Beissbarth Jemima J Hare Kim M KM Kirkham Lea-Ann S LA Smith-Vaughan Heidi H
Journal of clinical microbiology 20131204 2
We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials. ...[more]
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