Ontology highlight
ABSTRACT:
SUBMITTER: Pfitzner C
PROVIDER: S-EPMC3913920 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Pfitzner Claudia C Schröder Isabel I Scheungraber Cornelia C Dogan Askin A Runnebaum Ingo Bernhard IB Dürst Matthias M Häfner Norman N
Scientific reports 20140205
The detection of circulating tumour cells (CTC) in cancer patients may be useful for therapy monitoring and prediction of relapse. A sensitive assay based on HPV-oncogene transcripts which are highly specific for cervical cancer cells was established. The Digital-Direct-RT-PCR (DD-RT-PCR) combines Ficoll-separation, ThinPrep-fixation and one-step RT-PCR in a low-throughput digital-PCR format enabling the direct analysis and detection of individual CTC without RNA isolation. Experimental samples ...[more]