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One-step RT-droplet digital PCR: a breakthrough in the quantification of waterborne RNA viruses.

ABSTRACT: Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR), and needs no standard curve. This new tool is fully amenable for the quantification of viruses in the particularly low concentrations usually found in water samples.

SUBMITTER: Racki N

PROVIDER: S-EPMC3892107 | biostudies-other | 2014 Jan

REPOSITORIES: biostudies-other

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One-step RT-droplet digital PCR: a breakthrough in the quantification of waterborne RNA viruses.

Rački Nejc N Morisset Dany D Gutierrez-Aguirre Ion I Ravnikar Maja M

Analytical and bioanalytical chemistry 20131126 3

Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR) ...[more]

PMID: 24276251

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