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Cytoplasmic 5'-3' exonuclease Xrn1p is also a genome-wide transcription factor in yeast.

ABSTRACT: The 5' to 3' exoribonuclease Xrn1 is a large protein involved in cytoplasmatic mRNA degradation as a critical component of the major decaysome. Its deletion in the yeast Saccharomyces cerevisiae is not lethal, but it has multiple physiological effects. In a previous study, our group showed that deletion of all tested components of the yeast major decaysome, including XRN1, results in a decrease in the synthetic rate and an increase in half-life of most mRNAs in a compensatory manner. Furthermore, the same study showed that the all tested decaysome components are also nuclear proteins that bind to the 5' region of a number of genes. In the present work, we show that disruption of Xrn1 activity preferentially affects both the synthesis and decay of a distinct subpopulation of mRNAs. The most affected mRNAs are the transcripts of the highly transcribed genes, mainly those encoding ribosome biogenesis and translation factors. Previously, we proposed that synthegradases play a key role in regulating both mRNA synthesis and degradation. Evidently, Xrn1 functions as a synthegradase, whose selectivity might help coordinating the expression of the protein synthetic machinery. We propose to name the most affected genes "Xrn1 synthegradon."

SUBMITTER: Medina DA

PROVIDER: S-EPMC3915102 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Cytoplasmic 5'-3' exonuclease Xrn1p is also a genome-wide transcription factor in yeast.

Medina Daniel A DA Jordán-Pla Antonio A Millán-Zambrano Gonzalo G Chávez Sebastián S Choder Mordechai M Pérez-Ortín José E JE

Frontiers in genetics 20140206

The 5' to 3' exoribonuclease Xrn1 is a large protein involved in cytoplasmatic mRNA degradation as a critical component of the major decaysome. Its deletion in the yeast Saccharomyces cerevisiae is not lethal, but it has multiple physiological effects. In a previous study, our group showed that deletion of all tested components of the yeast major decaysome, including XRN1, results in a decrease in the synthetic rate and an increase in half-life of most mRNAs in a compensatory manner. Furthermore ...[more]

PMID: 24567736

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Cytoplasmic 5'-3' exonuclease Xrn1p is also a genome-wide transcription factor in yeast.

Publications

Cytoplasmic 5'-3' exonuclease Xrn1p is also a genome-wide transcription factor in yeast.

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