Ontology highlight
ABSTRACT:
SUBMITTER: Dickey SW
PROVIDER: S-EPMC3917317 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Dickey Seth W SW Baker Rosanna P RP Cho Sangwoo S Urban Siniša S
Cell 20131201 6
Enzymatic cleavage of transmembrane anchors to release proteins from the membrane controls diverse signaling pathways and is implicated in more than a dozen diseases. How catalysis works within the viscous, water-excluding, two-dimensional membrane is unknown. We developed an inducible reconstitution system to interrogate rhomboid proteolysis quantitatively within the membrane in real time. Remarkably, rhomboid proteases displayed no physiological affinity for substrates (K(d) ~190 μM/0.1 mol%). ...[more]