Unknown

Dataset Information

0

Vertical nanowire probes for intracellular signaling of living cells.


ABSTRACT: The single living cell action potential was measured in an intracellular mode by using a vertical nanoelectrode. For intracellular interfacing, Si nanowires were vertically grown in a controlled manner, and optimum conditions, such as diameter, length, and nanowire density, were determined by culturing cells on the nanowires. Vertical nanowire probes were then fabricated with a complimentary metal-oxide-semiconductor (CMOS) process including sequential deposition of the passivation and electrode layers on the nanowires, and a subsequent partial etching process. The fabricated nanowire probes had an approximately 60-nm diameter and were intracellular. These probes interfaced with a GH3 cell and measured the spontaneous action potential. It successfully measured the action potential, which rapidly reached a steady state with average peak amplitude of approximately 10 mV, duration of approximately 140 ms, and period of 0.9 Hz.

SUBMITTER: Lee KY 

PROVIDER: S-EPMC3917366 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Vertical nanowire probes for intracellular signaling of living cells.

Lee Ki-Young KY   Kim Ilsoo I   Kim So-Eun SE   Jeong Du-Won DW   Kim Ju-Jin JJ   Rhim Hyewhon H   Ahn Jae-Pyeong JP   Park Seung-Han SH   Choi Heon-Jin HJ  

Nanoscale research letters 20140203 1


The single living cell action potential was measured in an intracellular mode by using a vertical nanoelectrode. For intracellular interfacing, Si nanowires were vertically grown in a controlled manner, and optimum conditions, such as diameter, length, and nanowire density, were determined by culturing cells on the nanowires. Vertical nanowire probes were then fabricated with a complimentary metal-oxide-semiconductor (CMOS) process including sequential deposition of the passivation and electrode  ...[more]

Similar Datasets

| S-EPMC3910633 | biostudies-literature
| S-EPMC7281467 | biostudies-literature
| S-EPMC4209482 | biostudies-literature
| S-EPMC3946362 | biostudies-literature
| S-EPMC7062878 | biostudies-literature
| S-EPMC3303933 | biostudies-literature
| S-EPMC6057472 | biostudies-literature
| S-EPMC7146857 | biostudies-literature
| S-EPMC5193178 | biostudies-literature
| S-EPMC2862989 | biostudies-literature