Ontology highlight
ABSTRACT:
SUBMITTER: Kuchenov D
PROVIDER: S-EPMC5193178 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Cell chemical biology 20161208 12
Essential characteristics of cellular signaling networks include a complex interconnected architecture and temporal dynamics of protein activity. The latter can be monitored by Förster resonance energy transfer (FRET) biosensors at a single-live-cell level with high temporal resolution. However, these experiments are typically limited to the use of a couple of FRET biosensors. Here, we describe a FRET-based multi-parameter imaging platform (FMIP) that allows simultaneous high-throughput monitori ...[more]