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Preservation of H? production activity in nanoporous latex coatings of Rhodopseudomonas palustris?CGA009 during dry storage at ambient temperatures.


ABSTRACT: To assess the applicability of latex cell coatings as an 'off-the-shelf' biocatalyst, the effect of osmoprotectants, temperature, humidity and O? on preservation of H? production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24?h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H? production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H? production activity, whereas considerable H? production was still detected in sucrose- and trehalose-stabilized coatings. The relative humidity level at which the coatings were stored had a significant impact on the recovery and subsequent rates of H? production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H? (0-0.1% headspace accumulation), whereas those stored at

SUBMITTER: Piskorska M 

PROVIDER: S-EPMC3918154 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Preservation of H₂ production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures.

Piskorska M M   Soule T T   Gosse J L JL   Milliken C C   Flickinger M C MC   Flickinger M C MC   Smith G W GW   Yeager C M CM  

Microbial biotechnology 20130701 5


To assess the applicability of latex cell coatings as an 'off-the-shelf' biocatalyst, the effect of osmoprotectants, temperature, humidity and O₂ on preservation of H₂ production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H₂ production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H₂ production  ...[more]

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