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ABSTRACT:
SUBMITTER: Tabassum R
PROVIDER: S-EPMC3918720 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Tabassum Romana R Khaliq Shazia S Rajoka Muhammad Ibrahim MI Agblevor Foster F
Biotechnology research international 20140121
The thermodynamic and kinetic properties of solids state raw starch digesting alpha amylase from newly isolated Bacillus licheniformis RT7PE1 strain were studied. The kinetic values Q p , Y p/s , Y p/X , and q p were proved to be best with 15% wheat bran. The molecular weight of purified enzyme was 112 kDa. The apparent K m and V max values for starch were 3.4 mg mL(-1) and 19.5 IU mg(-1) protein, respectively. The optimum temperature and pH for α -amylase were 55°C, 9.8. The half-life of enzyme ...[more]