Ontology highlight
ABSTRACT:
SUBMITTER: Ohdan K
PROVIDER: S-EPMC92111 | biostudies-literature | 2000 Jul
REPOSITORIES: biostudies-literature
Ohdan K K Kuriki T T Takata H H Kaneko H H Okada S S
Applied and environmental microbiology 20000701 7
We constructed two types of chimeric enzymes, Ch1 Amy and Ch2 Amy. Ch1 Amy consisted of a catalytic domain of Bacillus subtilis X-23 alpha-amylase (Ba-S) and the raw starch-binding domain (domain E) of Bacillus A2-5a cyclomaltodextrin glucanotransferase (A2-5a CGT). Ch2 Amy consisted of Ba-S and D (function unknown) plus E domains of A2-5a CGT. Ch1 Amy acquired raw starch-binding and -digesting abilities which were not present in the catalytic part (Ba-S). Furthermore, the specific activity of C ...[more]