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Mini-blaster-mediated targeted gene disruption and marker complementation in Candida albicans.

ABSTRACT: Several gene disruption strategies have been described in Candida albicans to create homozygous mutants. We describe here a recyclable mini-blaster cassette containing C. albicans URA3 gene and 200-bp flanking repeats that is useful for disruption of C. albicans genes. The cassette can be used to create unmarked homozygous mutants which can be complemented at the HIS1 gene locus. This strategy of creating gene disruptions and subsequent complementation can be used to study gene function.

SUBMITTER: Ganguly S 

PROVIDER: S-EPMC3918946 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Mini-blaster-mediated targeted gene disruption and marker complementation in Candida albicans.

Ganguly Shantanu S   Mitchell Aaron P AP  

Methods in molecular biology (Clifton, N.J.) 20120101

Several gene disruption strategies have been described in Candida albicans to create homozygous mutants. We describe here a recyclable mini-blaster cassette containing C. albicans URA3 gene and 200-bp flanking repeats that is useful for disruption of C. albicans genes. The cassette can be used to create unmarked homozygous mutants which can be complemented at the HIS1 gene locus. This strategy of creating gene disruptions and subsequent complementation can be used to study gene function. ...[more]

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