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IFN?-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.

ABSTRACT: Dendritic cells (DCs) release exosomes with different characteristics based on stimulus. Here, we showed that DC cultures stimulated with low-level IFN? released exosomes (IFN?-DC-Exos) that contained microRNA species that can increase baseline myelination, reduce oxidative stress, and improve remyelination following acute lysolecithin-induced demyelination. Furthermore, nasally administered IFN?-DC-Exos increased CNS myelination in vivo. IFN?-DC-Exos were preferentially taken up by oligodendrocytes, suggesting that they directly impact oligodendrocytes to increase myelination. Thus, our results show great potential for use of these IFN?-DC-Exos as a therapeutic to promote remyelination in multiple sclerosis and dysmyelinating syndromes.

SUBMITTER: Pusic AD

PROVIDER: S-EPMC3920591 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.

Pusic Aya D AD Pusic Kae M KM Clayton Benjamin L L BL Kraig Richard P RP

Journal of neuroimmunology 20131109 1-2

Dendritic cells (DCs) release exosomes with different characteristics based on stimulus. Here, we showed that DC cultures stimulated with low-level IFNγ released exosomes (IFNγ-DC-Exos) that contained microRNA species that can increase baseline myelination, reduce oxidative stress, and improve remyelination following acute lysolecithin-induced demyelination. Furthermore, nasally administered IFNγ-DC-Exos increased CNS myelination in vivo. IFNγ-DC-Exos were preferentially taken up by oligodendroc ...[more]

PMID: 24275061

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