Ontology highlight
ABSTRACT:
SUBMITTER: Tan BH
PROVIDER: S-EPMC3921932 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Tan Boon Hooi BH Chor Leow Thean T Foo Hooi Ling HL Abdul Rahim Raha R
BioMed research international 20140127
A superoxide dismutase (SOD) gene of Lactococcus lactis M4 was cloned and expressed in a prokaryotic system. Sequence analysis revealed an open reading frame of 621 bp which codes for 206 amino acid residues. Expression of sodA under T7 promoter exhibited a specific activity of 4967 U/mg when induced with 1 mM of isopropyl-β-D-thiogalactopyranoside. The recombinant SOD was purified to homogeneity by immobilised metal affinity chromatography and Superose 12 gel filtration chromatography. Sodium d ...[more]