Ontology highlight
ABSTRACT:
SUBMITTER: Santos N
PROVIDER: S-EPMC3923520 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20130613 7
Bacterial translation termination is mediated by release factors RF1 and RF2, which recognize stop codons and catalyze hydrolysis of the peptidyl-tRNA ester bond. The catalytic mechanism has been debated. We proposed that the backbone amide NH group, rather than the side chain, of the glutamine of the universally conserved GGQ motif participates in catalysis by H-bonding to the tetrahedral transition-state intermediate and by product stabilization. This was supported by complete loss of RF1 cata ...[more]