Ontology highlight
ABSTRACT:
SUBMITTER: Rupachandra S
PROVIDER: S-EPMC3925513 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Rupachandra S S Sarada D V L DV
BioMed research international 20140130
A 35 KDa protein referred to as F3 was purified from the seeds of Borreria hispida by precipitation with 80% ammonium sulphate and gel filtration on Sephadex G-100 column. RP-HPLC analysis of protein fraction (F3) on an analytical C-18 column produced a single peak, detected at 220 nm. F3 showed an apparent molecular weight of 35 KDa by SDS PAGE and MALDI-TOF-MS analyses. Peptide mass fingerprinting analysis of F3 showed the closest homology with the sequence of 1-aminocyclopropane-1-carboxylate ...[more]