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Studies on optimization of growth parameters for L-asparaginase production by Streptomyces ginsengisoli.

ABSTRACT: A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by "shake flask" method. The quantity of L-asparaginase produced was estimated as 3.23 ? mol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30 °C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.

SUBMITTER: Deshpande N

PROVIDER: S-EPMC3925603 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Studies on optimization of growth parameters for L-asparaginase production by Streptomyces ginsengisoli.

Deshpande Neelima N Choubey Prachi P Agashe Manasi M

TheScientificWorldJournal 20140129

A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by "shake flask" method. The quantity of L-asparaginase produced was estimated as 3.23 μ mol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various cond ...[more]

PMID: 24616652

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