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Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis.

ABSTRACT: Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin trafficking toward the cleavage furrow during cytokinesis. Our results thus identify a novel mechanism for fusion inhibition in mitosis and implicate its role in vesicle trafficking after anaphase onset.

SUBMITTER: Ikawa K

PROVIDER: S-EPMC3925722 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis.

Ikawa Keisuke K Satou Ayaka A Fukuhara Mitsuko M Matsumura Shigeru S Sugiyama Naoyuki N Goto Hidemasa H Fukuda Mitsunori M Inagaki Masaki M Ishihama Yasushi Y Toyoshima Fumiko F

Cell cycle (Georgetown, Tex.) 20131025 1

Endocytic vesicle fusion is inhibited during mitosis, but the molecular pathways that mediate the inhibition remain unclear. Here we uncovered an essential role of Polo-like kinase 1 (Plk1) in this mechanism. Phosphoproteomic analysis revealed that Plk1 phosphorylates the intermediate filament protein vimentin on Ser459, which is dispensable for its filament formation but is necessary for the inhibition of endocytic vesicle fusion in mitosis. Furthermore, this mechanism is required for integrin ...[more]

PMID: 24196446

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