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Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis.


ABSTRACT: Several kinases phosphorylate vimentin, the most common intermediate filament protein, in mitosis. Aurora-B and Rho-kinase regulate vimentin filament separation through the cleavage furrow-specific vimentin phosphorylation. Cdk1 also phosphorylates vimentin from prometaphase to metaphase, but its significance has remained unknown. Here we demonstrated a direct interaction between Plk1 and vimentin-Ser55 phosphorylated by Cdk1, an event that led to Plk1 activation and further vimentin phosphorylation. Plk1 phosphorylated vimentin at approximately 1 mol phosphate/mol substrate, which partly inhibited its filament forming ability, in vitro. Plk1 induced the phosphorylation of vimentin-Ser82, which was elevated from metaphase and maintained until the end of mitosis. This elevation followed the Cdk1-induced vimentin-Ser55 phosphorylation, and was impaired by Plk1 depletion. Mutational analyses revealed that Plk1-induced vimentin-Ser82 phosphorylation plays an important role in vimentin filaments segregation, coordinately with Rho-kinase and Aurora-B. Taken together, these results indicated a novel mechanism that Cdk1 regulated mitotic vimentin phosphorylation via not only a direct enzyme reaction but also Plk1 recruitment to vimentin.

SUBMITTER: Yamaguchi T 

PROVIDER: S-EPMC2171270 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature

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Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis.

Yamaguchi Tomoya T   Goto Hidemasa H   Yokoyama Tomoya T   Silljé Herman H   Hanisch Anja A   Uldschmid Andreas A   Takai Yasushi Y   Oguri Takashi T   Nigg Erich A EA   Inagaki Masaki M  

The Journal of cell biology 20051031 3


Several kinases phosphorylate vimentin, the most common intermediate filament protein, in mitosis. Aurora-B and Rho-kinase regulate vimentin filament separation through the cleavage furrow-specific vimentin phosphorylation. Cdk1 also phosphorylates vimentin from prometaphase to metaphase, but its significance has remained unknown. Here we demonstrated a direct interaction between Plk1 and vimentin-Ser55 phosphorylated by Cdk1, an event that led to Plk1 activation and further vimentin phosphoryla  ...[more]

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